Purification of the 43 kDa glycoprotein from exocellular components excreted by Paracoccidioides brasiliensis in liquid culture (TOM medium)

Abstract

The 43 kDa glycoprotein (gp43) antigen has been purified by affinity chromatography and Sephacryl S-200 gel filtration from concentrated and dialysed culture supernatant fluids of Paracoccidioides brasiliensis grown in a tomato juice-enriched complex medium (TOM). In the TOM medium, there was rapid and extensive growth of the fungus, with an abundant production of gp43 in relation to other exocellularly accumulated components. The purified gp43 can be used for immunochemical and structure determination studies.