The effect of acidosis on the relationship between Ca2+ and force in isolated ferret cardiac muscle

Abstract

1. The relationship between force and intracellular [Ca2+] (monitored using the protein aequorin) has been investigated in papillary muscles isolated from ferret hearts, under control conditions (superfusate pH (pHo) 7.3) and during acidosis (pHo 6.8). 2. At pHo 7.3, increasing bathing [Ca2+] from 0.5 mmol l-1 to 8 mmol l-1 led to an increase in the size of the intracellular calcium transient. At the lower [Ca2+] this was accompanied by an increase in developed force; however, at the higher bathing [Ca2+] developed force reached a plateau. 3. Acidosis (produced by increasing the [CO2] of the gas with which the muscle superfusate was equilibrated) decreased maximum force and shifted the curve relating peak developed force to peak intracellular [Ca2+] to the right. 4. The mechanisms underlying the apparent decrease in the sensitivity of the contractile proteins to Ca2+ were investigated by applying rapid length changes to papillary muscles at control pHo, during acidosis, and after bathing [Ca2+] had been increased to match force during acidosis to that in control. 5. Acidosis decreased the change in force produced in response to a given length change (i.e. decreased muscle stiffness) but when bathing [Ca2+] was increased during acidosis, muscle stiffness returned to control. 6. Acidosis had no effect on muscle stiffness after the induction of rigor in the muscle (produced by metabolic inhibition). 7. It is suggested that in intact cardiac muscle the major effect of a mild acidosis is to decrease the sensitivity of the contractile proteins to Ca2+, hence decreasing the number of bound cross-bridges.