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. 1991 Jun 25;19(12):3345-50.
doi: 10.1093/nar/19.12.3345.

A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays

J Van Ness  1 , S Kalbfleisch, C R Petrie, M W Reed, J C Tabone, N M Vermeulen
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Free PMC article

A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays

J Van Ness et al. Nucleic Acids Res. .
Free PMC article

Abstract

A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.

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