Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jul 1;88(13):5680-4.
doi: 10.1073/pnas.88.13.5680.

Hypoxia-inducible nuclear factors bind to an enhancer element located 3' to the human erythropoietin gene

G L Semenza  1 , M K Nejfelt, S M Chi, S E Antonarakis
Affiliations

Hypoxia-inducible nuclear factors bind to an enhancer element located 3' to the human erythropoietin gene

G L Semenza et al. Proc Natl Acad Sci U S A. .

Abstract

Human erythropoietin gene expression in liver and kidney is inducible by anemia or hypoxia. DNase I-hypersensitive sites were identified 3' to the human erythropoietin gene in liver nuclei. A 256-base-pair region of 3' flanking sequence was shown by DNase I protection and electrophoretic mobility-shift assays to bind four or more different nuclear factors, at least two of which are induced by anemia in both liver and kidney, and the region functioned as a hypoxia-inducible enhancer in transient expression assays. These results provide insight into the molecular basis for the regulation of gene expression by a fundamental physiologic stimulus, hypoxia.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed
    1. Proc Soc Exp Biol Med. 1957 Jan;94(1):237-41 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7580-4 - PubMed
    1. Cell. 1986 Feb 28;44(4):565-76 - PubMed
    1. Mol Cell Biol. 1986 Jul;6(7):2571-5 - PubMed

Publication types

LinkOut - more resources