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. 2010 Jul 14:10:369.
doi: 10.1186/1471-2407-10-369.

Synergistic effect of stromelysin-1 (matrix metalloproteinase-3) promoter (-1171 5A->6A) polymorphism in oral submucous fibrosis and head and neck lesions

Ajay K Chaudhary  1 , Mamta Singh, Alok C Bharti, Mangal Singh, Shirish Shukla, Atul K Singh, Ravi Mehrotra
Affiliations

Synergistic effect of stromelysin-1 (matrix metalloproteinase-3) promoter (-1171 5A->6A) polymorphism in oral submucous fibrosis and head and neck lesions

Ajay K Chaudhary et al. BMC Cancer. .

Abstract

Background: Matrix metalloproteinases (MMPs) are enzymes that degrade all the components of extra cellular matrix and collagen. Various types of MMPs are known to be expressed and activated in patients with oral submucous fibrosis (OSMF) as well as head and neck squamous cell carcinoma (HNSCC). The purpose of this study was to asses the association of the single nucleotide polymorphism (SNP) adenosine insertion/deletion polymorphism (-1171 5A->6A) in the MMP-3 promoter region in these lesions.

Methods: MMP-3 SNP was genotyped by polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) analysis in a case control study consisting of 362 participants; 101 cases of OSMF, 135 of HNSCC and 126 controls, compared for age, sex and habits. ROC distribution was plotted to assess the contributions of genetic variation in MMP-3 genotypes with relation to age.

Results: Analysis of MMP 3 (-1171 5A->6A) polymorphism revealed the frequency of 5A allele in OSMF, HNSCC and controls to be 0.15, 0.13 and 0.07, respectively. A significant difference was found in 5A genotype frequency between OSMF (5A genotype frequency = 0.15, p = 0.01, OR = 2.26, 95% CI = 1.22-4.20) and in controls (5A genotype frequency 0.07) as well as HNSCC (5A genotype frequency 0.13, p = 0.03,95%CI = 1.06-3.51) and controls (5A genotype frequency = 0.07) In this study, 5A genotype had greater than two fold risk for developing OSMF (OR = 2.26) and nearly the same in case of HNSCC (OR = 1.94) as compared to controls. In patients with OSMF as well as HNSCC, the ROC analysis between the MMP-3 genotype and age, 6A/6A allele was found to be significant in patients both over and under 45 years of age; while the 5A/5A carrier alleles showed an association only in patients less than 45 years of age.

Conclusions: This study concluded that the expression of MMP-3 genotype associated with the 5A alleles, it may have an important role in the susceptibility of the patients to develop OSMF and HNSCC.

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Figures

Figure 1
Figure 1
A The expected PCR product size of MMP-3 gene by 100 bp marker [M], Lane 1-7 shows PCR product followed by separation on 2.0% agarose gel was confirmed; B: Genotyping of MMP-3 Promoter (-1171 5A->6A) Polymorphism by PCR-RFLP analysis followed by separation on 3. 5% agarose gel, Lane M = 100 bp marker; lane 1, 3, 4, 5 = 5A/6A; lane 2 = 5A/6A; lane 6 = 5A/5A.
Figure 2
Figure 2
Clinical picture of patients with OSMF and HNSCC.
Figure 3
Figure 3
ROC 6A/6A allele was also found to be significant in subjects both over and under 45 years of age, while 5A/5A carrier alleles showed an association only in patients less than 45 years of age in case of [A] OSMF and [B] HNSCC.
See this image and copyright information in PMC

References

    1. Vihinen P, Kähäri VM. Matrix metalloproteinases in cancer: prognostic markers and therapeutic targets. Int J Cancer. 2002;99(2):157–66. doi: 10.1002/ijc.10329. - DOI - PubMed
    1. Matrisian LM. Cancer biology. Extracellular proteinases in malignancy. Curr Biol. 1999;9:776–778. doi: 10.1016/S0960-9822(00)80011-1. - DOI - PubMed
    1. Impola U, Uitto VJ, Hietanen J, Hakkinen L, Zhang L, Larjava H, Isaka K, Saarialho-Kere U. Differential expression of matrilysin-1 (MMP-7), 92 kD gelatinase (MMP-9), and metalloelastase (MMP-12) in oral verrucous and squamous cell cancer. J Pathol. 2004;202(1):14–22. doi: 10.1002/path.1479. - DOI - PubMed
    1. Brinckerhoff CE, Rutter JL, Benbow U. Interstitial collagenases as markers of tumor progression. Clin Cancer Res. 2000;6(12):4823–4830. - PubMed
    1. Johanssona N, Ahonenb M, Kähäri VM. Matrix metalloproteinases in tumor invasion. Cell Mol Life Sci. 2000;57:5–15. doi: 10.1007/s000180050495. - DOI - PMC - PubMed

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