Location of cells giving phase-locked responses to pure tones in the primary auditory cortex

Abstract

Phase-locked responses to pure tones have previously been described in the primary auditory cortex (AI) of the guinea pig. They are interesting because they show that some cells may use a temporal code for representing sounds of 60-300 Hz rather than the rate or place mechanisms used over most of AI. Our previous study had shown that the phase-locked responses were grouped together, but it was not clear whether they were in separate minicolumns or a larger macrocolumn. We now show that the phase-locked cells are arranged in a macrocolumn within AI that forms a subdivision of the isofrequency bands. Phase-locked responses were recorded from 158 multiunits using silicon based multiprobes with four shanks. The phase-locked units gave the strongest response in layers III/IV but phase-locked units were also recorded in layers II, V and VI. The column included cells with characteristic frequencies of 80 Hz-1.3 kHz (0.5-0.8 mm long) and was about 0.5 mm wide. It was located at a constant position at the intersection of the coronal plane 1 mm caudal to bregma and the suture that forms the lateral edge of the parietal bone.