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Comparative Study
. 2010 Sep;17(9):1481-3.
doi: 10.1128/CVI.00223-10. Epub 2010 Jul 14.

Utility of pandemic H1N1 2009 influenza virus recombinant hemagglutinin protein-based enzyme-linked immunosorbent assay for serosurveillance

V A Arankalle  1 , R G Virkar, B V Tandale, N B Ingle
Affiliations
Comparative Study

Utility of pandemic H1N1 2009 influenza virus recombinant hemagglutinin protein-based enzyme-linked immunosorbent assay for serosurveillance

V A Arankalle et al. Clin Vaccine Immunol. 2010 Sep.

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG antibodies against the pandemic H1N1 2009 influenza A virus, employing a recombinant hemagglutinin protein of the virus, was compared to the hemagglutination inhibition (HI) test using 783 serum samples. The results showed a concordance of 98.4%, suggesting the utility of the ELISA in serosurveillance. Two hundred sixty-nine (100%) serum samples with an HI titer of > or =20 were ELISA reactive.

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Figures

FIG. 1.
FIG. 1.
Relationship of log HI and ELISA titers. Each point represents the mean positive ELISA titer for a given HI titer value. Error bars represent standard errors of the means.
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