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. 2010:2010:519589.
doi: 10.1155/2010/519589. Epub 2010 Jun 16.

Upregulation of TLR2/4 expression in mononuclear cells in postoperative systemic inflammatory response syndrome after liver transplantation

Ziqing Hei  1 , Xinjin Chi, Nan Cheng, Gangjian Luo, Shangrong Li
Affiliations

Upregulation of TLR2/4 expression in mononuclear cells in postoperative systemic inflammatory response syndrome after liver transplantation

Ziqing Hei et al. Mediators Inflamm. 2010.

Abstract

Background: To explore the relationship between Toll-like rpheral blood mononuclear cells (PBMC) and systemic inflammatory response syndrome (SIRS) in postoperative patients of liver transplantation (LT).

Methods: Blood samples of 27 patients receiving LT were collected at T1 (after induction of anaesthesia), T2 (25 minutes after the beginning of anhepatic phase), T3 (3 hours after graft reperfusion), and T4 (24 hours after graft reperfusion). The expression of TLR2/4 on PBMC and serum concentration of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-8 were measured. The patients were divided into SIRS group (n = 12) and non-SIRS group (n = 15) for analysis.

Results: Blood loss and transfusion were higher in the SIRS group than in the non-SIRS group. Both the preanhepatic and anhepatic phase were significantly longer in the SIRS group. The TLR2/4 expression on PBMC as well as serum TNF-alpha, IL-1beta, and IL-8 were significantly higher at T3 and T4 than that at T1 and T2 in the SIRS patients. The expression of TLR4 on PBMC is positively correlated to serum TNF-alpha, IL-8. Expression of TLR2/4 on PBMC and serum concentrations of TNF-alpha, IL-1beta, did not differ among the 4-time points in non-SIRS patients.

Conclusions: Upregulation of TLR2/4 expression on PBMC may contribute to the development of postoperative SIRS during perioperative period of LT.

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Figures

Figure 1
Figure 1
Representative FACS plots of TLR2 staining on PBMC. (a) SIRS; (b) non-SIRS. The non-specific binding is relatively small relative to specific binding as defined by the isotype controls. Dotted line represents the isotype control.
Figure 2
Figure 2
Representative FACS plots of TLR4 staining on PBMC. (a) SIRS; (b) non-SIRS. The non-specific binding is relatively small relative to specific binding as defined by the isotype controls. Dotted line represents the isotype control.
Figure 3
Figure 3
Upregulation of TLR2 expression on PBMC. Mean ± SD (n = 12 for SIRS; n = 15 for non-SIRS); *P < .01,  # P < .05, compared with T1/T1.
Figure 4
Figure 4
Upregulation of TLR4 expression on PBMC. Mean ± SD (n = 12 for SIRS; n = 15 for non-SIRS); *P < .01,  # P < .05, compared with T1/T1.
Figure 5
Figure 5
Increase of serum TNF-α. Median (Q) n = 12 for SIRS; (n = 15 for non-SIRS); *P < .05, compared with T1/T1.
Figure 6
Figure 6
Increase of serum IL-1β. Median (Q) (n = 12 for SIRS; n = 15 for non-SIRS); *P < .05, compared with T1/T1.
Figure 7
Figure 7
Increase of serum IL-8. Median (Q) (in SIRS group, n = 12; in non-SIRS group, n = 15); *P < .05, compared with T1/T1.
See this image and copyright information in PMC

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