[Purification of calf thymus adenosine diphosphate ribose polymerase]
- PMID: 206372
[Purification of calf thymus adenosine diphosphate ribose polymerase]
Abstract
Calf thymus poly ADPR polymerase has been purified to electrophoretic homogenity. The enzyme has a molecular weight of 120,000 +/- 10,000 dalton. The substrate affinity is very high (apparent Km 82.5 micrometer). The presence of exogenous DNA does not appear to be a requisite for enzymatic activity of the purified enzyme.