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Comparative Study
. 2010 Sep;28(9):1568-70.
doi: 10.1002/stem.471.

Human induced pluripotent stem cells develop teratoma more efficiently and faster than human embryonic stem cells regardless the site of injection

Ivan Gutierrez-Aranda, Veronica Ramos-Mejia, Clara Bueno, Martin Munoz-Lopez, Pedro J Real, Angela Mácia, Laura Sanchez, Gertrudis Ligero, Jose L Garcia-Parez, Pablo Menendez
Free PMC article
Comparative Study

Human induced pluripotent stem cells develop teratoma more efficiently and faster than human embryonic stem cells regardless the site of injection

Ivan Gutierrez-Aranda et al. Stem Cells. 2010 Sep.
Free PMC article
No abstract available

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Figures

Figure 1
Figure 1
Human iPSCs form teratomas faster and with higher efficiency than hESCs regardless the site of injection. (A): Cartoon summarizing the experimental design. (B): Table summarizing the efficiency, latency, and histological analysis of the teratomas developed from both human ESCs and human iPSCs upon either subcutaneous or intratesticular transplantation. (C): Histological analysis of teratomas derived from both hESCs and iPSCs upon subcutaneous or intratesticular transplantation reveal similar tissue composition. The presence of mesodermal tissues is defined by teratoma sections containing cartilage and positive for both smooth muscle actin and melanin. The presence of ectodermal tissues was scored as teratoma sections that stained positive for β-III tubulin and presence of neural rosettes. Endoderm was defined as teratoma sections that stained positive for α-fetoprotein and pan-cytokeratin. The study was approved by the Andalusian and Spanish Embryo and Cellular Reprogramming Ethical Institutional review Board. Animal protocols were approved by the Local University Hospital Council On Animal Care and Experimentation. Abbreviations: ESCs, embryonic stem cells; iPSCs, reprogrammed/induced pluripotent stem cells.
See this image and copyright information in PMC

References

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