Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2010 Jul 16;329(5989):285-7.
doi: 10.1126/science.1192224.

Biochemistry. Reengineering enzymes

Stefan Lutz  1
Affiliations
Comment

Biochemistry. Reengineering enzymes

Stefan Lutz. Science. .
No abstract available

PubMed Disclaimer

Figures

Figure
Figure
Tailoring enzymes to specific reactions. (A) Savile et al. reprogrammed the substrate specificity of a transaminase to perform a reaction in the industrial production of an antidiabetes drug, sitagliptin. Mutational hot spots shown in red are in the active site, and those shown in yellow stabilize binding of the dimeric form of the active enzyme. (B) Siegel et al. designed an enzyme to perform the Diels-Alder reaction between two molecules based on first principles. They computer-generated a large ensemble of structures in which two key amino acids in the active site (depicted in red and blue) are bound to the transition state. These structures were fitted to a protein scaffold, and target enzymes were then designed and synthesized.
See this image and copyright information in PMC

Comment on

References

    1. Siegel JB, et al. Science. 2010;329:309. - PMC - PubMed
    1. Savile CK, et al. Science. 2010;329:305. - PubMed
    1. Hilvert D, et al. J. Am. Chem. Soc. 1989;111:9261.
    1. Seelig B, Jäschke A. Chem. Biol. 1999;6:167. - PubMed
    1. Tarasow TM, et al. Nature. 1997;389:54. - PubMed

Publication types

MeSH terms