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. 2010 Jul 20;5(7):e11625.
doi: 10.1371/journal.pone.0011625.

Reduced NAA-levels in the NAWM of patients with MS is a feature of progression. A study with quantitative magnetic resonance spectroscopy at 3 Tesla

Fahmy Aboul-Enein  1 , Martin Krssák, Romana Höftberger, Daniela Prayer, Wolfgang Kristoferitsch
Affiliations

Reduced NAA-levels in the NAWM of patients with MS is a feature of progression. A study with quantitative magnetic resonance spectroscopy at 3 Tesla

Fahmy Aboul-Enein et al. PLoS One. .

Abstract

Background: Reduced N-acetyl-aspartate (NAA) levels in magnetic resonance spectroscopy (MRS) may visualize axonal damage even in the normal appearing white matter (NAWM). Demyelination and axonal degeneration are a hallmark in multiple sclerosis (MS).

Objective: To define the extent of axonal degeneration in the NAWM in the remote from focal lesions in patients with relapsing-remitting (RRMS) and secondary progressive MS (SPMS).

Material and methods: 37 patients with clinical definite MS (27 with RRMS, 10 with SPMS) and 8 controls were included. We used 2D (1)H-MR-chemical shift imaging (TR = 1500ms, TE = 135ms, nominal resolution 1ccm) operating at 3Tesla to assess the metabolic pattern in the fronto-parietal NAWM. Ratios of NAA to creatine (Cr) and choline (Cho) and absolute concentrations of the metabolites in the NAWM were measured in each voxel matching exclusively white matter on the anatomical T2 weighted MR images.

Results: No significant difference of absolute concentrations for NAA, Cr and Cho or metabolite ratios were found between RRMS and controls. In SPMS, the NAA/Cr ratio and absolute concentrations for NAA and Cr were significantly reduced compared to RRMS and to controls.

Conclusions: In our study SPMS patients, but not RRMS patients were characterized by low NAA levels. Reduced NAA-levels in the NAWM of patients with MS is a feature of progression.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Spectral region including resonance lines of choline (Cho), creatine (Cr) and N-acetyl-aspartate (NAA).
Left column, anatomical T2w MR Images with the depiction of MRSI PRESS box volume of interest (grey, 8×8 cm) and voxels (white) in the NAWM chosen for the spectroscopic data evaluation. Right column, representative MR spectra from a single voxel with original spectra (lower trace), AMARES estimate (second trace), individual components (third trace) and fit residue (upper trace). Data from RRMS (upper part) and SSPMS (middle part) patients as well as healthy controls (CON, lower part) are presented.
Figure 2
Figure 2. MRS Ratios of brain metabolite in NAWM of MS patients and controls.
white, NAA/Cr ratio; grey, NAA/Cho ratios; black, Cho/Cr ratios; squares, RRMS patients (n = 27); circles, SPMS patients (n = 10); triangles, controls (n = 8); bars, means.
Figure 3
Figure 3. MRS absolute concentrations of brain metabolite in NAWM of MS patients and controls.
white, NAA [mM]; grey, Cr [mM]; black, Cho [mM]. squares, RRMS patients (n = 27); circles, SPMS patients (n = 10); triangles, controls (n = 8); bars, means.
See this image and copyright information in PMC

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