Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Sep 3;4(12):1469-74.
doi: 10.1007/s11671-009-9422-7.

A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies

Hao Yang  1 , Qing Guo, Rong He, Ding Li, Xueqing Zhang, Chenchen Bao, Hengyao Hu, Daxiang Cui
Affiliations

A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies

Hao Yang et al. Nanoscale Res Lett. .

Abstract

Quantum dot is a special kind of nanomaterial composed of periodic groups of II-VI, III-V or IV-VI materials. Their high quantum yield, broad absorption with narrow photoluminescence spectra and high resistance to photobleaching, make them become a promising labeling substance in biological analysis. Here, we report a quick and parallel analytical method based on quantum dots for ToRCH-related antibodies including Toxoplasma gondii, Rubella virus, Cytomegalovirus and Herpes simplex virus type 1 (HSV1) and 2 (HSV2). Firstly, we fabricated the microarrays with the five kinds of ToRCH-related antigens and used CdTe quantum dots to label secondary antibody and then analyzed 100 specimens of randomly selected clinical sera from obstetric outpatients. The currently prevalent enzyme-linked immunosorbent assay (ELISA) kits were considered as "golden standard" for comparison. The results show that the quantum dots labeling-based ToRCH microarrays have comparable sensitivity and specificity with ELISA. Besides, the microarrays hold distinct advantages over ELISA test format in detection time, cost, operation and signal stability. Validated by the clinical assay, our quantum dots-based ToRCH microarrays have great potential in the detection of ToRCH-related pathogens.

Keywords: Antibody; Diagnosis; Microarray; Quantum dots; Serology.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The rationale of QDs-based microarray
Figure 2
Figure 2
Schematic representation of microarray
Figure 3
Figure 3
TEM image of prepared QDs
Figure 4
Figure 4
Fluorescence spectra of QDs solution before and after labeling with goat-anti-human IgG (inset: images of QDs before and after the coupling with second antibodies under UV irradiation)
Figure 5
Figure 5
The microarray results with corresponding control sera (anegative serum;bpositive control serum of Toxoplasmosis;cpositive control serum of Cytomegalovirus;dpositive control serum of Rubella virus;epositive control serum of Herpes simplex virus type 1;fpositive control serum of Herpes simplex virus type 2)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. van ‘t Veer LJ, Dai H, van de Vijver MJ, He YD, Hart AAM, Mao M, Peterse HL, van der Kooy K, Marton MJ, Witteveen AT, Schreiber GJ, Kerkhoven RM, Roberts C, Linsley PS, Bernards R, Friend SH. Nature. 2002. p. 530. - DOI - PubMed
    1. Inoue H, Matsuyama A, Mimori K, Ueo H, Mori M, Chong JM, Fukayama M, Kodama T, Aburatani H. Clin. 2002. p. 3475. COI number [1:CAS:528:DC%2BD38XpsVCgtLw%3D] - PubMed
    1. Wang H-Y, Greenawalt D, Cui X, Tereshchenko IV, Luo M, Yang Q, Azaro MA, Hu G, Chu Y, Li JY, Shen L, Lin Y, Zhang L, Li H. J. 2009. p. 6. - DOI - PMC - PubMed
    1. Volchenboum SL, Li C, Li S, Attiyeh EF, Patrick Reynolds C, Maris JM, Thomas Look A, George RE. Cancer Res. 2009. p. 4143. COI number [1:CAS:528:DC%2BD1MXlvFWltrc%3D] - DOI - PMC - PubMed
    1. Du W, Ma X, Nyman D, Povlsen K, Akguen N, Schneider EM. Diagn. 2007. p. 355. COI number [1:CAS:528:DC%2BD2sXhsVWjsLvO] - DOI - PubMed

LinkOut - more resources