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. 2010 May 25;15(5):3694-708.
doi: 10.3390/molecules15053694.

Optimization of alkaline extraction of polysaccharides from Ganoderma lucidum and their effect on immune function in mice

Sheng-Quan Huang  1 , Jin-Wei Li, Zhou Wang, Hua-Xin Pan, Jiang-Xu Chen, Zheng-Xiang Ning
Affiliations

Optimization of alkaline extraction of polysaccharides from Ganoderma lucidum and their effect on immune function in mice

Sheng-Quan Huang et al. Molecules. .

Abstract

Response surface methodology was employed to optimize the conditions for alkaline extraction of polysaccharides from Ganoderma lucidum. The results indicated that the optimum conditions were an extraction temperature of 60.1 degrees C, an extraction time of 77.3 min, a sodium hydroxide (NaOH) concentration of 5.1% and a substrate/liquid ratio of 1:21.4. Immunological assays results have shown that the alkaline soluble polysaccharides have no noticeable effects on monocyte phagocytosis and immune organ (spleen, thymus) weight of immunocompromised mice at the tested dosages. However, they could restore delayed type hypersensitivity reaction to dinitrofluorobenzene (DFNB), hemolysis antibody levels at the three doses applied, and improve the natural killer cell activity at the high-dose and medium dose.

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Figures

Figure 1
Figure 1
The surface and contour plot of polysaccharide yield as affected by extraction temperature (X1), extraction time (X2), NaOH concentration (X3) and ratio of liquid to solid (X4). Where (A) is X1 and X3 (liquid/solid ratio 20:1,time 80 min); (B) is X3 and X2(alkali/solid ratio 20:1,temperature 60 °C); (C) is X4 and X3 (temperature 60 °C, time 80min); (D) is X3 and X2 (NaOH 5.0%,time 80 min).,(E) is X4 and X2(temperature 60 °C, NaOH 5.0%) ;(F) is X1 and X2 (liquid/solid ratio 20:1, time 80 min).
Figure 2
Figure 2
SEM micrograph of microwave/ultrasound assisted extraction residue at 1,000-fold magnification.
Figure 3
Figure 3
SEM micrograph of alkaline extraction residue at 1,000-fold magnification.
See this image and copyright information in PMC

References

    1. Franz G. Polysaccharides in pharmacy: current applications and future concepts. Planta Med. 1989;55:493–497. doi: 10.1055/s-2006-962078. - DOI - PubMed
    1. Furusawa E., Chou S.C., Furusawa S., Hirazami A., Dang Y. Antitumor activity of Ganoderma lucidum, an edible mushroom, on intraperitoneally implanted Lewis Lung Carcinoma in synergenic mice. Phytother. Res. 1992;6:300–304. doi: 10.1002/ptr.2650060604. - DOI
    1. Shiao M.S., Lee K.R., Lin L.J., Wang C.T. Natural products and biological activities of the Chinese medical fungus, Ganoderma lucidum. In: Ho C.T., Osawa T., Huang M.T., Rosen R.T., editors. Food Phytochemicals for Cancer Prevention Ⅱ: Teas, Spices, and Herbs. American Chemical Society; Washington, DC, USA: 1994. pp. 342–354.
    1. Li Y.Q., Fang L., Zhang K.C. Structure and bioactivities of a galactose rich extracellular polysaccharide from submergedly cultured Ganoderma lucidum. Carbohydr. Polym. 2007;68:323–328.
    1. Kim Y.S., Eo S.K., Oh K.W., Lee Ch.k., Han S.S. Antiherpetic activities of acidic protein bound polysaccharide isolated from Ganoderma lucidum alone and in combinations with interferons. J. Ethnopharmacol. 2000;72:451–458. - PubMed

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