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. 1991 Jul;173(14):4537-9.
doi: 10.1128/jb.173.14.4537-4539.1991.

The hemimethylated replication origin of Escherichia coli can be initiated in vitro

E Boye  1
Affiliations

The hemimethylated replication origin of Escherichia coli can be initiated in vitro

E Boye. J Bacteriol. 1991 Jul.

Abstract

Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.

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