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. 1991 Jul;173(14):4540-3.
doi: 10.1128/jb.173.14.4540-4543.1991.

Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters

M J Feldhaus  1 , V Hwa, Q Cheng, A A Salyers
Affiliations

Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters

M J Feldhaus et al. J Bacteriol. 1991 Jul.

Abstract

We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.

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