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. 2010 Sep;24(9):1662-4.
doi: 10.1038/leu.2010.156. Epub 2010 Jul 29.

Limited engraftment of low-risk myelodysplastic syndrome cells in NOD/SCID gamma-C chain knockout mice

M G Martin, J S Welch, G L Uy, T A Fehniger, S Kulkarni, E J Duncavage, M J Walter

Limited engraftment of low-risk myelodysplastic syndrome cells in NOD/SCID gamma-C chain knockout mice

M G Martin et al. Leukemia. 2010 Sep.
No abstract available

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Figures

Figure 1
Figure 1
Engraftment of human bone marrow cells into NSG mice. 5 × 105 – 5 × 106 total bone marrow cells (5 × 106 cells for AML samples), 1.8–5 × 106 T-cell depleted bone marrow cells, or 5 × 104 – 2 × 106 CD34+ selected cells were administered to NSG mice by retro-orbital (RO) injection, or 3.3–8 × 105 total bone marrow cells administered by intra-tibial (IT) injection. Engraftment was assessed by flow cytometry of bone marrow cells at 7 – 12 weeks post-xenografting for murine CD45, human CD45, human CD3, CD19 and CD33. A. Percentage of human CD45+ cells in bone marrow cells at 7 – 12 weeks post-xenografting. B. Percentage of human CD33+ cells in bone marrow cells at 7 – 12 weeks post-xenografting. C. Percentage of human CD3+ cells in bone marrow cells at 7 – 12 weeks post-xenografting. D. Immunohistochemistry of xenograft lung tissue stained with anti-human CD3ε (clone F7.2.38, Dako) consistent with GVHD. E. Immunohistochemistry of xenograft liver tissue stained with anti-human CD3 consistent with GVHD. Blast %= percent of myeloblast in the bone marrow.
See this image and copyright information in PMC

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