Interleukin-6 contributes to age-related alteration of cytokine production by macrophages

Abstract

Here, we studied in vitro cytokine production by splenic macrophages obtained from young and aged BALB/c wild type (WT) and IL-6 knockout (IL-6 KO) mice. Relative to macrophages obtained from young WT mice given lipopolysaccharide (LPS), those from aged WT mice had decreased production of proinflammatory cytokines. In contrast, when compared to macrophages from young IL-6 KO mice, LPS stimulation yielded higher levels of these cytokines by cells from aged IL-6 KO mice. Aging or IL-6 deficiency did not affected the percentage of F4/80(+) macrophages, or the surface expression of Toll-like receptor 4 (TLR4) and components of the IL-6 receptor. Overall, our results indicate that IL-6 plays a role in regulating the age-related defects in macrophages through alteration of proinflammatory cytokines, adding to the complexity of IL-6-mediated impairment of immune cell function with increasing age.

Figure 1

Splenic macrophages obtained from young and aged WT and IL-6 KO mice were cultured for 18 hours with LPS (100 ng/ml). Supernatants collected were assayed for TNFα (a), IL-1β (b), IL-6 (c), and IL-12 (d), by ELISA. Data are shown as mean ± SEM of 4-5 mice per group. *P < .05 from young WT. ^# P < .05 from young WT. ^† P < .05 from young IL-6 KO. ^@ P < .05 from aged WT.