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Review
. 2010 Aug 3;122(5):517-26.
doi: 10.1161/CIRCULATIONAHA.109.881441.

Stem cell therapy for vascular regeneration: adult, embryonic, and induced pluripotent stem cells

Nicholas J Leeper  1 , Arwen L Hunter, John P Cooke
Affiliations
Review

Stem cell therapy for vascular regeneration: adult, embryonic, and induced pluripotent stem cells

Nicholas J Leeper et al. Circulation. .
No abstract available

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Figures

Figure 1
Figure 1
Embryonic stem cells, adult stem cells and induced pluripotential stem cells. The three progenitor cell types available for vascular regenerative therapy- their promise and peril.
Figure 2
Figure 2
Characterization of iPSCs. iPSCs derived from human fibroblast reprogrammed with lentiviral vectors expressing Oct-3/4, Sox2 and Klf4 maintain colony morphology characteristic of ESC (A: 4X, B: 20X), demonstrate alkaline phosphatase activity (C) and express pluripotency markers as detected by immunocytochemistry including Nanog (D), TRA-1-81 (E) and SSEA-4 (F). For panels D-F pluripotency markers were detected using antibodies tagged by Alexa-594 (red) and nuclei are marked by dapi staining (blue). Notably, control plates of differentiated fibroblasts that were mock transfected failed to give rise to any hESC-like colonies.
Figure 3
Figure 3
Characterization of human iPSC-derived ECs. iPSCs were differentiated and selected for expression of CD34 by magnetic sorting. (A) CD34+ cells contain a population that demonstrates expression of PECAM-1/CD31 by FACS analysis. EC phenotype of these iPSC-derived CD31+/CD34+ cells is confirmed by expression of EC markers (B) PECAM-1/CD31 and (C) VE-Cadherin, (D) morphological appearance under phase contrast microscopy and by manifestations of EC function including (E) tube formation in matrigel.
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