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. 1991 Jul 15;88(14):6210-3.
doi: 10.1073/pnas.88.14.6210.

Phosphatidate-dependent protein phosphorylation

S B Bocckino  1 , P B Wilson, J H Exton
Affiliations

Phosphatidate-dependent protein phosphorylation

S B Bocckino et al. Proc Natl Acad Sci U S A. .

Abstract

Phosphatidate-dependent protein phosphorylation was observed in soluble extracts from rat liver, brain, lung, and testis. The phosphorylation was stimulated by free Ca2+ in the range of 360-800 nM. Incubation mixtures containing phosphatidate provided markedly different profiles of protein phosphorylation from those with phosphatidylserine plus 1,2-diolein. Phosphatidate-dependent phosphorylation of a 30-kDa protein in the soluble fraction from heart was also observed. This phosphorylation did not require Ca2+. Soluble fractions from liver, testis, brain, and lung phosphorylated the 30-kDa heart protein in a phosphatidate-dependent Ca(2+)-independent manner. We propose that part of the action of phosphatidate in cells may be mediated by a protein kinase(s).

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