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Comparative Study
. 2010 Aug;83(2):314-8.
doi: 10.4269/ajtmh.2010.09-0790.

ELISA versus conventional methods of diagnosing endemic brucellosis

Basappa Mantur  1 , Aisha Parande, Satish Amarnath, Giridhar Patil, Ravindra Walvekar, Arun Desai, Mahantesh Parande, Rupali Shinde, Masiyappa Chandrashekar, Satish Patil
Affiliations
Comparative Study

ELISA versus conventional methods of diagnosing endemic brucellosis

Basappa Mantur et al. Am J Trop Med Hyg. 2010 Aug.

Abstract

The diagnostic value of enzyme-linked immunosorbent assay (ELISA) was evaluated when blood specimens of 92 patients suspected of brucellosis underwent the ELISA (IgM and IgG), standard tube agglutination (SAT), and 2-mercaptoethanol (2-ME) tests and blood cultures; 38 sera from non-brucellosis patients and 34 sera from blood donors were also subjected to ELISA, SAT, and 2-ME tests. SAT was able to pinpoint only 23 (25%), whereas ELISA confirmed the etiology in 56 (60.9%; P < 0.001) patients with brucellosis, including 31 culture-confirmed cases. The sensitivity and specificity of ELISA were 100% and 71.31%, respectively. Because they were confirmed by ELISA, the diagnosis could never be excluded with SAT in 33 cases. ELISA has been found to be more sensitive in acute (28% higher sensitivity; P < 0.02) and chronic (55% higher sensitivity; P < 0.01) cases. For accurate diagnosis in suspected brucellosis cases detection, we recommend both ELISA IgM and IgG tests. ELISA IgG and 2-ME tests seem to be promising tools in judging prognosis.

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References

    1. Sohn AH, Probert WS, Glaser CA, Gupta N, Bollen AW, Wong JD, Grace EM, McDonald WC. Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp. Emerg Infect Dis. 2003;9:485–488. - PMC - PubMed
    1. McDonald WL, Jamaludin R, Mackereth G, Hansen M, Humphrey S, Short P, Taylor T, Swingler J, Dawson CE, Whatmore AM, Stabberfield E, Perrett LL, Simmons G. Characterization of a Brucella spp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand. J Clin Microbiol. 2006;44:4363–4370. - PMC - PubMed
    1. Wallach JC, Miguel SE, Baldi PC, Guarnera E, Goldbaum FA, Fossati CA. Urban outbreak of a Brucella melitensis infection in an Argentine family: clinical and diagnostic aspects. FEMS Immunol Med Microbiol. 1994;8:49–56. - PubMed
    1. Young EJ. Human brucellosis. Rev Infect Dis. 1983;5:821–842. - PubMed
    1. Maas KS, Mendez M, Zavaleta M, Manrique J, Franco MP, Mulder M, Bonifacio N, Castaneda ML, Chacaltana J, Yagui E, Gilman RH, Guillen A, Blazes DL, Espinosa B, Hall E, Abdoel TH, Smits HL. Evaluation of brucellosis by PCR and persistence after treatment in patients returning to the hospital for follow-up. Am J Trop Med Hyg. 2007;76:698–702. - PubMed

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