Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Mar;75(3):1510-4.
doi: 10.1073/pnas.75.3.1510.

Repertoire of antiviral antibodies expressed by somatic cell hybrids

W Gerhard, C M Croce, D Lopes, H Koprowski

Repertoire of antiviral antibodies expressed by somatic cell hybrids

W Gerhard et al. Proc Natl Acad Sci U S A. 1978 Mar.

Abstract

Fusion between P3 x 63 Ag8 mouse myeloma cells and spleen cells from BALB/c mice immunized with influenza type A or B or parainfluenza type 1 virus generated reproducibly antiviral antibody-producing somatic cell hybrids (hybridomas). Eleven hybridomas derived from spleen cells of mice immunized with influenza type A virus were directed against the viral hemagglutinin, one reacted with a host component derived from chickens, and one expressed a specificity not further characterized. The hybridoma antibodies tended to be highly specific for the hemagglutinin of the immunizing virus and seemed to express the same repertoire of strain-specific antibody reactivities as splenic precursor B cells, they did not express any of the frequently occurring crossreactive anti-hemagglutinin specificities. Hybridomas producing crossreactive antibodies against hemagglutinin could be obtained if priming and boosting virus were heterologous.

PubMed Disclaimer

References

    1. Science. 1964 Aug 14;145(3633):709-10 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8 - PubMed
    1. J Exp Med. 1978 Jan 1;147(1):1-12 - PubMed
    1. J Exp Med. 1976 Oct 1;144(4):985-95 - PubMed
    1. Nature. 1977 Apr 7;266(5602):550-2 - PubMed

Publication types

LinkOut - more resources