Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Sep;7(9):717-9.
doi: 10.1038/nmeth.1489. Epub 2010 Aug 8.

Live-cell super-resolution imaging with trimethoprim conjugates

Richard Wombacher  1 , Meike Heidbreder, Sebastian van de Linde, Michael P Sheetz, Mike Heilemann, Virginia W Cornish, Markus Sauer
Affiliations

Live-cell super-resolution imaging with trimethoprim conjugates

Richard Wombacher et al. Nat Methods. 2010 Sep.

Abstract

The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at approximately 20 nm resolution.

PubMed Disclaimer

References

    1. J Microsc. 2010 Jan;237(1):12-22 - PubMed
    1. Nat Methods. 2008 Feb;5(2):159-61 - PubMed
    1. Biophys J. 2002 May;82(5):2775-83 - PubMed
    1. Chemphyschem. 2010 Mar 15;11(4):836-40 - PubMed
    1. Nat Methods. 2005 Apr;2(4):255-7 - PubMed

Publication types

LinkOut - more resources