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. 2010 Sep;7(9):729-32.
doi: 10.1038/nmeth.1488. Epub 2010 Aug 8.

Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano

Kazuki Horikawa  1 , Yoshiyuki Yamada, Tomoki Matsuda, Kentarou Kobayashi, Mitsuhiro Hashimoto, Toru Matsu-ura, Atsushi Miyawaki, Takayuki Michikawa, Katsuhiko Mikoshiba, Takeharu Nagai
Affiliations
Free article

Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano

Kazuki Horikawa et al. Nat Methods. 2010 Sep.
Free article

Abstract

We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.

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References

    1. Microbiol Immunol. 2006;50(8):643-54 - PubMed
    1. J Cell Biol. 2006 Jun 5;173(5):755-65 - PubMed
    1. Nat Protoc. 2006;1(3):1235-47 - PubMed
    1. Methods. 2008 Nov;46(3):143-51 - PubMed
    1. Nat Protoc. 2006;1(3):1057-65 - PubMed