Alterations in intracellular calcium transients of fibroblasts from progressive systemic sclerotic patients: a digital imaging microscopic study

Abstract

Intracellular Ca2+ concentrations ([Ca2+]i) in cultured skin fibroblasts from normal subjects and progressive systemic sclerosis (PSS) patients were determined by using Fura-2 and fluorescent videomicroscopy. With the exception of fibroblasts from one PSS patient showing a higher [Ca2+]i, no significant difference was observed in resting [Ca2+]i between the two groups of fibroblasts. Bradykinin (BK) (10 microM) induced a transient [Ca2+]i increase in normal fibroblasts, whereas the BK-induced [Ca2+]i increase was reduced or not detectable in PSS fibroblasts. Removal of extracellular Ca2+ did not eliminate the BK-induced [Ca2+]i increase in normal fibroblasts. These findings suggest that BK stimulates Ca2+ release from intracellular stores in human fibroblasts, and also that the BK-mediated Ca2+ release is impaired in PSS fibroblasts.