doi: 10.1126/science.206966.
Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents
- PMID: 206966
- DOI: 10.1126/science.206966
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Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents
Science.
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Abstract
Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).
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