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. 1978 Jun 9;200(4346):1168-70.
doi: 10.1126/science.206966.

Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents

W S Hancock, C A Bishop, R L Prestidge, D R Harding, M T Hearn

Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents

W S Hancock et al. Science. .

Abstract

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).

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