Complete nucleotide sequence of KPC-3-encoding plasmid pKpQIL in the epidemic Klebsiella pneumoniae sequence type 258

Abstract

We have determined the entire DNA sequence of plasmid pKpQIL, the bla(KPC-3)-carrying plasmid harbored by the carbapenem-resistant Klebsiella pneumoniae clone sequence type 258 (ST 258) in Israel. pKpQIL is a 113,637-bp, self-transmissible plasmid that belongs to the incompatibility group IncFII. It consists of a large backbone of a pKPN4-like plasmid and carries the bla(KPC-3)-containing Tn4401a transposon of a pNYC-like plasmid.

FIG. 1.

Genetic map of the full-length, 113-kb pKpQIL plasmid. The arrows indicate gene location and orientation. Predicted ORFs with known functions are indicated by black arrows. ORFs of hypothetical proteins are indicated by white arrows. Boundaries of Tn4401 are marked with a bracket. The plasmid map was drawn using Redasoft visual cloning software. The black arc inside the circle shows the region in common with plasmid pKPN4 of K. pneumoniae MGH 78578, and the gray arc indicates the region in common with plasmid pNYC of K. pneumoniae strain YC.