doi: 10.1021/ja1052544.
Pulling genetic RNA out of tobacco mosaic virus using single-molecule force spectroscopy
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- PMID: 20698668
- DOI: 10.1021/ja1052544
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Pulling genetic RNA out of tobacco mosaic virus using single-molecule force spectroscopy
J Am Chem Soc.
.
Abstract
RNA-coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA-protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid-protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.
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