Anti-HCV reactive volunteer blood donors distribution character and genotypes switch in Xi'an, China

Abstract

HCV is prevailed in the world as well as in China. Blood transfusion is one of the most common transmission pathways of this pathogen. Although data of HCV infection character were reported during the past years, anti-HCV reactive profile of China donors was not fully clear yet. Furthermore, infection progress was found related to the HCV genotype. Different genotype led to different efficacy when interferon was introduced into HCV therapy. Here we provided character data of HCV infection in China blood donors from the year of 2000 to 2009. The infection rate in local donors was lower than general population and descended from 0.80% to 0.40% or so in recent years. About 83% HCV strains were categorized into genotypes 1b and 2a. But 1b subtype cases climbed and 2a subtype cases decreased. The current study threw more light on HCV infection of blood donors in China, at least in the Northern region.

Figure 1

HCV infection rate in blood donors from the year of 2000 to 2009. Donors' peripheral blood serum were isolated and employed to ELISA to test reactive anti-HCV antibody. Bars represent the 95% confidence interval.

Figure 2

HCV reactive profile of blood donors in represented (blank) and total samples (black). Represented and total samples from donors were administrated to standard ELISA, ALT and real-time PCR determination methods. The map was drawn according to different range respectively. A, S/CO value distribution of donors in anti-HCV antibody test; B, ALT value distribution of donors; C, viral load distribution of represented donors. E1:1 × 10¹; E2:1 × 10²; E3:1 × 10³; E4:1 × 10⁴; E5:1 × 10⁵.

Figure 3

Genotype distribution and switch in donors and patients. Blood sample form 200 represented donors and 100 patients were genotyped. Then genotype 1b and 2a cases were counted. After that, rate of 1b and 2a cases were distributed in different years. A, genotype 1b and 2a comparison in donors (blank) and patients (black); B, genotype 1b distribution from the year of 2000 to 2009; C genotype 2a distribution from the year of 2000 to 2009.

Figure 4

The scheme of genotyping of HCV from recruited volunteer donors. Samples were prepared and nested-PCR was performed. After that, serial restriction enzyme digestion was administrated to PCR products according to reports[7,8,20].