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. 2010 Oct;48(10):3758-61.
doi: 10.1128/JCM.00471-10. Epub 2010 Aug 11.

Multiplex amplified nominal tandem-repeat analysis (MANTRA), a rapid method for genotyping Mycobacterium tuberculosis by use of multiplex PCR and a microfluidic laboratory chip

Adam J Merritt  1 , Terillee Keehner, Lyn C O'Reilly, Russell L McInnes, Timothy J J Inglis
Affiliations

Multiplex amplified nominal tandem-repeat analysis (MANTRA), a rapid method for genotyping Mycobacterium tuberculosis by use of multiplex PCR and a microfluidic laboratory chip

Adam J Merritt et al. J Clin Microbiol. 2010 Oct.

Abstract

A variable-number tandem-repeat genotyping method for Mycobacterium tuberculosis was converted to run in a multiplex PCR format on a 12-well microfluidic laboratory chip. Epidemiologically and genotypically distinct isolate clusters of M. tuberculosis were identified. This rapid genotyping method has potential application in smaller clinical laboratories and public health field investigations.

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Figures

FIG. 1.
FIG. 1.
Gel-like image of MANTRA M. tuberculosis genotyping results, showing 34 analyzed samples and a DNA fragment size ladder. Upper and lower size markers are present in ladders and all M. tuberculosis isolate lanes. The vertical scale represents migration time.
FIG. 2.
FIG. 2.
Gel-like image of MANTRA M. tuberculosis genotyping results, showing a DNA fragment size ladder and 16 downselected samples. Isolate designation is shown at the top of each lane; group assignment is shown at the bottom. Upper and lower size markers are present in ladders and all M. tuberculosis isolate lanes. The vertical scale represents migration time.
See this image and copyright information in PMC

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