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. 1999 Dec;15(4):309-13.
doi: 10.1023/A:1008310617047.

A 4D TROSY-based pulse scheme for correlating 1HNi,15Ni,13Calphai,13C'i-1 chemical shifts in high molecular weight, 15N,13C, 2H labeled proteins

R Konrat  1 , D Yang, L E Kay
Affiliations

A 4D TROSY-based pulse scheme for correlating 1HNi,15Ni,13Calphai,13C'i-1 chemical shifts in high molecular weight, 15N,13C, 2H labeled proteins

R Konrat et al. J Biomol NMR. 1999 Dec.

Abstract

A 4D TROSY-based triple resonance experiment, 4D-HNCO(i-1)CA(i), is presented which correlates intra-residue (1)HN, (15)N, (13) C(alpha) chemical shifts with the carbonyl ((13)C') shift of the preceding residue. The experiment is best used in concert with recently described 4D TROSY-HNCOCA and -HNCACO experiments [Yang, D. and Kay, L.E. (1999) J. Am. Chem. Soc., 121, 2571-2575]. In cases where degeneracy of ((1)HN,(15)N) spin pairs precludes assignment using the HNCOCA and HNCACO, the HNCO(i-1)CA(i) often allows resolution of the ambiguity by linking the (13)C(alpha) and (13)C' spins surrounding the ((1)HN,(15)N) pair. The experiment is demonstrated on a sample of (15)N, (13)C, (2) H labeled maltose binding protein in complex with beta-cyclodextrin that tumbles with a correlation time of 46 ns.

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References

    1. Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12366-71 - PubMed
    1. J Biomol NMR. 1995 Nov;6(3):277-93 - PubMed
    1. J Biomol NMR. 1991 Nov;1(4):439-46 - PubMed
    1. J Biomol NMR. 1996 Jan;7(1):83-7 - PubMed
    1. Annu Rev Biophys Biomol Struct. 1998;27:357-406 - PubMed

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