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Comment
. 2010 Aug 24;107(34):14945-6.
doi: 10.1073/pnas.1009416107. Epub 2010 Aug 13.

RNA silencing amplification in plants: size matters

Rebecca Schwab  1 , Olivier Voinnet
Affiliations
Comment

RNA silencing amplification in plants: size matters

Rebecca Schwab et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Consequences of 21 vs. 22-nt small RNA binding to target transcripts. (A) An AGO1-associated 21-nt miRNA bound to a target transcript triggers target cleavage, but not the formation of 2° siRNAs from the 3′ cleavage fragment. Conversely, a 22-nt miRNA bound to AGO1 (B) recruits the RNA-dependent RNA polymerase RDR6 to generate double-stranded RNA from the 3′ cleavage fragment. Subsequent processing by DCL4 liberates 2° siRNAs. Although 22-nt miRNAs often result from single nt insertions in otherwise 2-nt miRNA duplexes that are generated by DCL1, DCL2 intrinsically produces 22-nt siRNAs from perfectly double-stranded templates (C). When associated with AGO1, 22-nt siRNAs might also recruit an RDR activity such that target transcripts (e.g., viral RNAs) would be destabilized not only by slicing via the primary (1°) siRNAs but also by a Dicer activity recruited to the newly formed double-stranded RNA.
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References

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