doi: 10.1039/c002155e.
Epub 2010 Mar 30.
A genetically encoded photocaged Nepsilon-methyl-L-lysine
Affiliations
- PMID: 20711534
- DOI: 10.1039/c002155e
Item in Clipboard
A genetically encoded photocaged Nepsilon-methyl-L-lysine
Mol Biosyst.
2010 Sep.
Abstract
A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.
Similar articles
-
The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives.Mol Biosyst. 2011 Mar;7(3):714-7. doi: 10.1039/c0mb00217h. Epub 2011 Jan 14. Mol Biosyst. 2011. PMID: 21234492
-
Recognition of non-alpha-amino substrates by pyrrolysyl-tRNA synthetase.J Mol Biol. 2009 Feb 6;385(5):1352-60. doi: 10.1016/j.jmb.2008.11.059. Epub 2008 Dec 11. J Mol Biol. 2009. PMID: 19100747
-
Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA.Science. 2002 May 24;296(5572):1459-62. doi: 10.1126/science.1069588. Science. 2002. PMID: 12029131
-
Adding amino acids to the genetic repertoire.Curr Opin Chem Biol. 2005 Dec;9(6):548-54. doi: 10.1016/j.cbpa.2005.10.011. Epub 2005 Nov 2. Curr Opin Chem Biol. 2005. PMID: 16260173 Review.
-
The direct genetic encoding of pyrrolysine.Curr Opin Microbiol. 2005 Dec;8(6):706-12. doi: 10.1016/j.mib.2005.10.009. Epub 2005 Oct 26. Curr Opin Microbiol. 2005. PMID: 16256420 Review.
Cited by
-
Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming.Chem Rev. 2024 Oct 9;124(19):11008-11062. doi: 10.1021/acs.chemrev.4c00243. Epub 2024 Sep 5. Chem Rev. 2024. PMID: 39235427 Free PMC article. Review.
-
Efficient cell factories for the production of N-methylated amino acids and for methanol-based amino acid production.Microb Biotechnol. 2022 Aug;15(8):2145-2159. doi: 10.1111/1751-7915.14067. Epub 2022 Apr 30. Microb Biotechnol. 2022. PMID: 35488805 Free PMC article. Review.
-
Genetic incorporation of seven ortho-substituted phenylalanine derivatives.ACS Chem Biol. 2014 Apr 18;9(4):884-90. doi: 10.1021/cb400917a. Epub 2014 Jan 27. ACS Chem Biol. 2014. PMID: 24451054 Free PMC article.
-
Chemical approaches to understand the language of histone modifications.ACS Chem Biol. 2011 Oct 21;6(10):987-99. doi: 10.1021/cb200142c. Epub 2011 Aug 17. ACS Chem Biol. 2011. PMID: 21827195 Free PMC article. Review.
-
Leveraging a Phage-Encoded Noncanonical Amino Acid: A Novel Pathway to Potent and Selective Epigenetic Reader Protein Inhibitors.ACS Cent Sci. 2024 Feb 28;10(4):782-792. doi: 10.1021/acscentsci.3c01419. eCollection 2024 Apr 24. ACS Cent Sci. 2024. PMID: 38680566 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
