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. 2010 Sep;6(9):1557-60.
doi: 10.1039/c002155e. Epub 2010 Mar 30.

A genetically encoded photocaged Nepsilon-methyl-L-lysine

Yane-Shih Wang  1 , Bo Wu, Zhiyong Wang, Ying Huang, Wei Wan, William K Russell, Pei-Jing Pai, Yin N Moe, David H Russell, Wenshe R Liu
Affiliations

A genetically encoded photocaged Nepsilon-methyl-L-lysine

Yane-Shih Wang et al. Mol Biosyst. 2010 Sep.

Abstract

A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

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