Rat thymic cultures: morphological and phenotypical characterization

Abstract

Rat thymic cultures have been established in order to analyse the morpho-functional characteristics of thymic epithelial and non-epithelial cells in vitro. Stromal cultures, originating from implanted thymic fragments, consist of fibroblasts occupying most of the culture surface, epithelial cells forming discrete colonies, thymocytes and bone marrow-derived cells. Epithelial cells show a low class II MHC antigen expression, which is highly increased in semi-adherent cells, and do not interact with thymocytes. Thymocytes proliferate extensively at the beginning of the culture, but almost disappear at the end of the first week; however, restarting of thymocyte proliferation occurs during the second week of culture. Bone marrow-derived cells include ED- Ia+ CR3- IL-2R- dendritic cells (DC), ED+ Ia+ CR3+ IL-2R+ non-adherent thymic phagocytic cells (PTR) and ED+ Ia- CR3- IL-2R- adherent type 1 and 2 macrophages, derived from PTR. Both PTR and DC establish lympho-stromatic complexes with thymocytes present in the cultures. These results suggest that PTR and DC present in rat thymic cultures belong to different cell lineages, and that they are, respectively, the in vitro equivalents of intrathymic macrophages and interdigitating cells.