Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2010 Sep;11(9):636-46.
doi: 10.1038/nrg2842.

Genome editing with engineered zinc finger nucleases

Fyodor D Urnov  1 , Edward J Rebar, Michael C Holmes, H Steve Zhang, Philip D Gregory
Affiliations
Review

Genome editing with engineered zinc finger nucleases

Fyodor D Urnov et al. Nat Rev Genet. 2010 Sep.

Abstract

Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and induced pluripotent stem cells, targeted integration in crop plants, and HIV resistance in immune cells - this broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair. Such 'genome editing' is now established in human cells and a number of model organisms, thus opening the door to a range of new experimental and therapeutic possibilities.

PubMed Disclaimer

References

    1. J Mol Biol. 2001 May 25;309(1):69-78 - PubMed
    1. Int Rev Immunol. 2010 Jun;29(3):235-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11163-7 - PubMed
    1. Nat Biotechnol. 2008 Jun;26(6):702-8 - PubMed
    1. Genome Res. 2010 Jan;20(1):81-9 - PubMed

LinkOut - more resources