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. 2010 Jul 7;11(7):2670-80.
doi: 10.3390/ijms11072670.

Molecular epidemiological study of pyrazinamide-resistance in clinical isolates of mycobacterium tuberculosis from South India

Muthuraj Muthaiah  1 , Sridharan Jagadeesan, Nisha Ayalusamy, Manupriya Sreenivasan, Sambamurthy Sangamesvara Prabhu, Usharani Muthuraj, Kamatchiyammal Senthilkumar, Saroja Veerappan
Affiliations

Molecular epidemiological study of pyrazinamide-resistance in clinical isolates of mycobacterium tuberculosis from South India

Muthuraj Muthaiah et al. Int J Mol Sci. .

Abstract

Pyrazinamide (PZA) has been in use for almost 50 years as a first-line drug for short-course chemotherapy against Mycobacterium tuberculosis. In this study, PCR mediated automated DNA sequencing is used to check the prevalence of PZA resistance among treatment failure cases of pulmonary tuberculosis. Out of 50 clinical isolates examined, 39 had mutations in the pncA gene that encodes Pyrazinamidase, an enzyme required to activate PZA. Of these, 31 (79.5%) were localized to three regions of pncA. We found two isolates with hitherto unreported mutation at amino acid 26 (Ala-->Gly) of pncA.

Keywords: PZA resistance; South-India; mycobacterium tuberculosis; pncA mutation; pulmonary tuberculosis.

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Figures

Figure 1.
Figure 1.
PCR Amplification for Species Identification. Lane 1: 100 bp DNA ladder, Lane 3:123 bp PCR amplified product.
Figure 2.
Figure 2.
PCR Amplification of pncA. Lane 1: 100 bp DNA ladder, Lane 3: 222 bp PCR amplified pncA gene product.
Figure 3.
Figure 3.
Electropherogram Analysis of PCR Amplified Products. Peak 1: Lower Marker (15 bp), Peak 2: PCR amplified product of pncA gene (222 bp) and Peak 3: Upper marker (1500 bp).
See this image and copyright information in PMC

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