Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Oct;48(10):3753-5.
doi: 10.1128/JCM.01466-10. Epub 2010 Aug 18.

Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens

P Lewis White  1 , Michael D Perry, Juergen Loeffler, Willem Melchers, Lena Klingspor, Stephane Bretagne, Elaine McCulloch, Manuel Cuenca-Estrella, Niklas Finnstrom, J Peter Donnelly, Rosemary A Barnes; European Aspergillus PCR Initiative
Affiliations

Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens

P Lewis White et al. J Clin Microbiol. 2010 Oct.

Abstract

A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.

PubMed Disclaimer

References

    1. De Pauw, B., T. J. Walsh, J. P. Donnelly, D. A. Stevens, J. E. Edwards, T. Calandra, P. G. Pappas, J. Maertens, O. Lortholary, C. A. Kauffman, D. W. Denning, T. F. Patterson, G. Maschmeyer, J. Bille, W. E. Dismukes, R. Herbrecht, W. W. Hope, C. C. Kibbler, B. J. Kullberg, K. A. Marr, P. Muñoz, F. C. Odds, J. R. Perfect, A. Restrepo, M. Ruhnke, B. H. Segal, J. D. Sobel, T. C. Sorrell, C. Viscoli, J. R. Wingard, T. Zaoutis, J. E. Bennett, European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group, and National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. 2008. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin. Infect. Dis. 46:1813-1821. - PMC - PubMed
    1. Herrera, M. L., A. C. Vallor, J. A. Gelfond, T. F. Patterson, and B. L. Wickes. 2009. Strain-dependent variation in 18S ribosomal DNA copy numbers in Aspergillus fumigatus. J. Clin. Microbiol. 47:1325-1332. - PMC - PubMed
    1. Loeffler, J., N. Henke, H. Hebart, D. Schmidt, L. Hagmeyer, U. Schumacher, and H. Einsele. 2000. Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system. J. Clin. Microbiol. 38:586-590. - PMC - PubMed
    1. Millon, L., R. Piarroux, E. Deconinck, C. E. Bulabois, F. Grenouillet, P. Rohrlich, J. M. Costa, and S. Bretagne. 2005. Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis. J. Clin. Microbiol. 43:5097-5101. - PMC - PubMed
    1. White, P. L., S. Bretagne, L. Klingspor, W. J. G. Melchers, E. McCulloch, B. Schulz, N. Finnstrom, C. Mengoli, R. A. Barnes, J. P. Donnelly, and J. Loeffler on behalf of the EuropeanAspergillus PCR Initiative. 2010. Aspergillus PCR: one step closer towards standardization. J. Clin. Microbiol. 48:1231-1240. - PMC - PubMed

MeSH terms