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. 2010 Sep;7(9):721-3.
doi: 10.1038/nmeth.1494. Epub 2010 Aug 22.

An antibiotic selection marker for nematode transgenesis

Rosina Giordano-Santini  1 , Stuart Milstein, Nenad Svrzikapa, Domena Tu, Robert Johnsen, David Baillie, Marc Vidal, Denis Dupuy
Affiliations

An antibiotic selection marker for nematode transgenesis

Rosina Giordano-Santini et al. Nat Methods. 2010 Sep.

Abstract

We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).

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Comment in

  • C. elegans select.
    Chamberlin HM. Chamberlin HM. Nat Methods. 2010 Sep;7(9):693-5. doi: 10.1038/nmeth0910-693. Nat Methods. 2010. PMID: 20805797

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