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. 2010 Aug 28;16(32):4089-94.
doi: 10.3748/wjg.v16.i32.4089.

Altered expression of MUC2 and MUC5AC in progression of colorectal carcinoma

Xiao-Dong Bu  1 , Nan Li, Xiao-Qiang Tian, Li Li, Jin-Song Wang, Xiao-Jin Yu, Pei-Lin Huang
Affiliations

Altered expression of MUC2 and MUC5AC in progression of colorectal carcinoma

Xiao-Dong Bu et al. World J Gastroenterol. .

Abstract

Aim: To study the expression profiles of MUC2 and MUC5AC in tumorigenesis of colorectal carcinoma and in its different pathologic types.

Methods: Formalin-fixed, paraffin-embedded human colorectal tissue specimens were immunostained with antibodies against MUC2 and MUC5AC. Six samples of normal mucosa (NM), 12 samples of hyperplastic polyp (HP), 15 samples of tubular adenoma with low-grade dysplasia (LGD), 14 samples of tubular adenoma with high-grade dysplasia (HGD), 26 samples of conventional colorectal adenocarcinoma (CCA), 15 samples of mucinous carcinoma (MC), and 8 samples of signet-ring cell carcinoma (SRCC) were collected.

Results: MUC2 was the most widely expressed protein in each study group, although the number of MUC2-positive cases was less in CCA group than in other groups (P < 0.05). The staining score for MUC2 was significantly decreased in the HP-LGD-HGD-CCA sequence (r = -0.73436, P < 0.0001). Among the neoplasms, MC and SRCC were more frequently associated with the high expression of MUC2 (P < 0.05) than with that of CCA. MUC5AC expression was detected in all groups but not in NM group. Furthermore, the staining score for MUC5AC was higher in HP, LGD, HGD, MC and SRCC groups than in NM and CCA groups (P < 0.05). The frequency of simultaneous expression of MUC proteins was significantly higher in MC and SRCC groups than in CCA group (P < 0.05).

Conclusion: Alterations in MUC expression occur during colorectal tumorigenesis. The transformation process in MC and SRCC may be different from that in the traditional adenoma-carcinoma sequence.

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Figures

Figure 1
Figure 1
Expression of MUC2 (SP method, × 200). A: Normal mucosa; B: Hyperplastic polyp; C: Tubular adenoma with low-grade dysplasia; D: Tubular adenoma with high-grade dysplasia; E: Conventional colorectal adenocarcinoma; F: Mucinous carcinoma; G: Signet-ring cell carcinoma.
Figure 2
Figure 2
Expression of MUC5AC (SP method, × 200). A: Normal mucosa; B: Hyperplastic polyp; C: Tubular adenoma with low-grade dysplasia; D: Tubular adenoma with high-grade dysplasia; E: Conventional colorectal adenocarcinoma; F: Mucinous carcinoma; G: Signet-ring cell carcinoma.
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References

    1. Andrianifahanana M, Moniaux N, Batra SK. Regulation of mucin expression: mechanistic aspects and implications for cancer and inflammatory diseases. Biochim Biophys Acta. 2006;1765:189–222. - PubMed
    1. Itoh Y, Kamata-Sakurai M, Denda-Nagai K, Nagai S, Tsuiji M, Ishii-Schrade K, Okada K, Goto A, Fukayama M, Irimura T. Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin. Glycobiology. 2008;18:74–83. - PubMed
    1. Kanoh A, Takeuchi H, Kato K, Waki M, Usami K, Irimura T. Interleukin-4 induces specific pp-GalNAc-T expression and alterations in mucin O-glycosylation in colonic epithelial cells. Biochim Biophys Acta. 2008;1780:577–584. - PubMed
    1. Komatsu K, Jono H, Lim JH, Imasato A, Xu H, Kai H, Yan C, Li JD. Glucocorticoids inhibit nontypeable Haemophilus influenzae-induced MUC5AC mucin expression via MAPK phosphatase-1-dependent inhibition of p38 MAPK. Biochem Biophys Res Commun. 2008;377:763–768. - PubMed
    1. Byrd JC, Bresalier RS. Mucins and mucin binding proteins in colorectal cancer. Cancer Metastasis Rev. 2004;23:77–99. - PubMed