doi: 10.1016/0005-2744(78)90109-2.
Pilot scale purification of alpha-galactosidase A from Cohn fraction IV-1 of human plasma
- PMID: 207338
- DOI: 10.1016/0005-2744(78)90109-2
Item in Clipboard
Pilot scale purification of alpha-galactosidase A from Cohn fraction IV-1 of human plasma
Biochim Biophys Acta.
.
Abstract
Human plasma alpha-galactosidase A (alpha-D-galactoside galactohydrolase, EC 3.2.1.22) was purified 7000-fold over plasma levels from Cohn Fraction IV-1. The yield per kg starting material averaged 11 000 units (nmol galactose liberated per h) and the specific activity was about 600 units per mg protein with 4-methylumbelliferyl-alpha-D-galactoside. The ratio of 4-methylumbelliferyl-alpha-galactosidase to ceramide trihexosidase activities was 6.2. Both activities were heat labile and exhibited the same relative mobilities on polyacrylamide gel electrophoresis. Enzymatic activity was stable for at least 4 months at 4 and -20 degrees C. The endotoxin concentration of this preparation averaged 0.26 mg per mg protein.
Similar articles
-
Plasma alpha-galactosidase A:properties and comparisons with tissue alpha-galactosidases.Biochim Biophys Acta. 1978 Aug 7;525(2):399-409. doi: 10.1016/0005-2744(78)90235-8. Biochim Biophys Acta. 1978. PMID: 210818
-
alpha-galactosidase A from human placenta. Stability and subunit size.Biochim Biophys Acta. 1977 Oct 13;484(2):408-16. doi: 10.1016/0005-2744(77)90096-1. Biochim Biophys Acta. 1977. PMID: 71921
-
Affinity purification of alpha-galactosidase A from human spleen, placenta, and plasma with elimination of pyrogen contamination. Properties of the purified splenic enzyme compared to other forms.J Biol Chem. 1981 Feb 10;256(3):1307-16. J Biol Chem. 1981. PMID: 6256390
-
[Microbial alpha-galactosidase (a review)].Prikl Biokhim Mikrobiol. 1982 Jan-Feb;18(1):3-15. Prikl Biokhim Mikrobiol. 1982. PMID: 6283520 Review. Russian.
-
Biotechnological potential of microbial α-galactosidases.Crit Rev Biotechnol. 2014 Dec;34(4):307-17. doi: 10.3109/07388551.2013.794124. Epub 2013 Aug 13. Crit Rev Biotechnol. 2014. PMID: 23937250 Review.
Cited by
-
Inherited X-chromosome inverted tandem duplication in a male traced to a grandparental mitotic error.Am J Hum Genet. 1986 May;38(5):741-50. Am J Hum Genet. 1986. PMID: 3459356 Free PMC article.
-
Overexpression of human alpha-galactosidase A results in its intracellular aggregation, crystallization in lysosomes, and selective secretion.J Cell Biol. 1992 Dec;119(5):1137-50. doi: 10.1083/jcb.119.5.1137. J Cell Biol. 1992. PMID: 1332979 Free PMC article.
-
Late-onset X-linked sideroblastic anemia. Missense mutations in the erythroid delta-aminolevulinate synthase (ALAS2) gene in two pyridoxine-responsive patients initially diagnosed with acquired refractory anemia and ringed sideroblasts.J Clin Invest. 1995 Oct;96(4):2090-6. doi: 10.1172/JCI118258. J Clin Invest. 1995. PMID: 7560104 Free PMC article.
-
First-trimester prenatal diagnosis of Tay-Sachs disease.Am J Hum Genet. 1984 Nov;36(6):1369-78. Am J Hum Genet. 1984. PMID: 6240199 Free PMC article.
-
Enzymatic defect in "X-linked" sideroblastic anemia: molecular evidence for erythroid delta-aminolevulinate synthase deficiency.Proc Natl Acad Sci U S A. 1992 May 1;89(9):4028-32. doi: 10.1073/pnas.89.9.4028. Proc Natl Acad Sci U S A. 1992. PMID: 1570328 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
