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. 2009 Feb;74(2):452-7.
doi: 10.1111/j.1095-8649.2008.02075.x.

In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio

A Babaryka  1 , E Kühn, R W Köster
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In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio

A Babaryka et al. J Fish Biol. 2009 Feb.

Abstract

The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.

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