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. 1997 Feb 26;119(8):2056-2057.
doi: 10.1021/ja963718g.

Difluorotoluene, a Nonpolar Isostere for Thymine, Codes Specifically and Efficiently for Adenine in DNA Replication

Sean Moran  1 , Rex X-F Ren, Squire Rumney, Eric T Kool
Affiliations

Difluorotoluene, a Nonpolar Isostere for Thymine, Codes Specifically and Efficiently for Adenine in DNA Replication

Sean Moran et al. J Am Chem Soc. .
No abstract available

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Figures

Figure 1
Figure 1
(A) Sequences of DNA template/primer duplexes in this study. Compound 1 is designated F (from difluorotoluene). (B) Products of “running-start” DNA synthesis on synthetic duplex templates containing analog 1 or thymidine at the sixth position downstream of the 18nt primer 3′ end. Autoradiogram of 15% denaturing polyacrylamide gel electrophoretic analysis of products of DNA synthesis, using a 32P-5′-end-labeled 18nt primer as shown in part A. Conditions are given in the text and in Supporting Information.
Figure 2
Figure 2
Histogram of fidelity of nucleotide insertion opposite T and isostere F (compound 1) with the 23nt primer template. Data are taken from Table 1.
See this image and copyright information in PMC

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