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Case Reports
. 2009 Oct 21;2(3):194-202.
doi: 10.1159/000249152.

Change of CD20 Expression in Diffuse Large B-Cell Lymphoma Treated with Rituximab, an Anti-CD20 Monoclonal Antibody: A Study of the Osaka Lymphoma Study Group

Naoki Wada  1 , Masaharu Kohara, Hiroyasu Ogawa, Haruo Sugiyama, Shirou Fukuhara, Yoichi Tatsumi, Akihisa Kanamaru, Masayuki Hino, Yuzuru Kanakura, Eiichi Morii, Katsuyuki Aozasa
Affiliations
Case Reports

Change of CD20 Expression in Diffuse Large B-Cell Lymphoma Treated with Rituximab, an Anti-CD20 Monoclonal Antibody: A Study of the Osaka Lymphoma Study Group

Naoki Wada et al. Case Rep Oncol. .

Abstract

Change of CD20 expression was examined in cases of diffuse large B-cell lymphoma (DLBCL). CD20 expression after treatment with anti-CD20 antibody (rituximab, Rx) for DLBCL was examined in 23 cases who received serial biopsy by immunohistochemistry (IHC) and flow cytometry (FCM). CD20- by IHC and/or FCM was defined as CD20-. Four cases were CD20- at initial biopsy but became CD20+ after chemotherapy with Rx (CH-R) (group A). Recurrent tumors in three group A cases became resistant to CH-R. Initial and recurrent tumors were CD20+ before and after CH-R in 17 cases (group B). Tumors before CH-R were CD20- in two cases (group C) and continued to be CD20- in one and turned CD20+ in the other with survival time after the relapse of 8 and 23 months, respectively. Evaluation of CD20 expression with immunohistochemical and flow cytometric methods is used for the prediction of responsiveness of relapsed DLBCL for CH-R.

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Figures

Fig. 1
Fig. 1
a Initial DLBCL (case 3). H&E. b Tumor cells were CD20+ at IHC. c Recurrent DLBCL after Rx treatment showing similar features to the initial DLBCL. H&E. d Recurrent tumor cells were CD20-. Inset Tumor cells were CD79a+. All ×400.
Fig. 2
Fig. 2
Polymerase chain reaction-based clonality analysis for Ig gene rearrangement (GeneScan analysis) in case 2 revealed the different peak pattern before and after Rx treatment with partial persistence of same sized peaks (↓).
See this image and copyright information in PMC

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