A comparison of five bioconjugatable ferrocenes for labeling of biomolecules

Abstract

Five electroactive ferrocene tags for labelling of biomolecules are contrasted with regard to conjugation reactivity with amine and thiol moieties, stability to loss of electrochemical activity, and impact of molecular structure on the redox potential of the free and DNA-conjugated forms.

Figure 1

(A) CV traces for solutions of FA0 in SPB as a function of storage time at 65 °C. Scan rate: 100 mV s⁻¹. (B) CV traces of FA0-DNA conjugates, pretreated in SPB at 65 °C for the indicated time, then hybridized to surface-bound complementary strands. Scan rate: 20 V s⁻¹.

Figure 2

Remnant electroactivity for ferrocene-DNA conjugates as a function of storage time at 65 °C.