Meta-analyses of effects of phytochemicals on digestibility and rumen fermentation characteristics associated with methanogenesis

Abstract

Background: A meta-analysis study was conducted to investigate the changes in rumen fermentation characteristics when methane inhibition by phytochemicals is employed. The whole database containing 185 treatment means from 36 published studies was divided into four subsets according to the major phytochemicals used in the studies, i.e. saponins, tannins, essential oils (EO) and organosulfur compounds (OS).

Results: Changes in protozoal numbers showed linear relationships with changes in methane production by saponins (R(2) = 0.48), tannins (R(2) = 0.30) and EO (R(2) = 0.20) but not OS. Concentrations of total volatile fatty acids (VFA) and acetate did not show any relationship (P > 0.1) with changes in methane due to saponins. However, propionate production increased linearly with increasing inhibition of methane (R(2) = 0.31), which resulted in a linear (R(2) = 0.26) decrease in acetate/propionate ratio (A/P) with decreasing methane production. Concentrations of total VFA, acetate and propionate did not change with changes in methane production by tannins. However, A/P showed a significant linear relationship (R(2) = 0.27) with decreasing methane formation. Concentrations of total VFA (R(2) = 0.44) and propionate (R(2) = 0.15) changed linearly and positively with changes in methane production by EO. However, acetate production (R(2) = 0.22) and A/P (R(2) = 0.17) increased linearly with increasing inhibition of methane by EO. Changes in concentrations of total VFA (R(2) = 0.60) and acetate (R(2) = 0.35) decreased linearly while those of propionate increased linearly (R(2) = 0.23) with increasing inhibition of methane by OS. Consequently, A/P decreased linearly (R(2) = 0.30) with decreasing methane production by OS. Digestibilities of organic matter (OM) and neutral detergent fibre were not affected by inhibition of methane production by saponins, EO and OS, but digestibility of OM decreased with decreasing methane production by tannins.

Conclusion: The inhibition of methane production by phytochemicals results in changes in rumen fermentation that differ depending on the types of phytochemicals.