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. 1990 Nov;24(3):208-17.
doi: 10.1016/0147-619x(90)90004-v.

Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2

A C Chang  1 , M B Slade, K L Williams
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Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2

A C Chang et al. Plasmid. 1990 Nov.

Abstract

Ddp2 is a 5.8-kb, high-copy-number, nuclear plasmid found in the eukaryote Dictyostelium discoideum. We have identified two functional domains, a large open reading frame (Rep gene) and a 626-bp fragment containing an origin of replication (ori). The ori, when cloned into a shuttle vector, confers stable extrachromosomal replication in D. discoideum, provided that the Rep gene, which acts in trans, is integrated into the host genome. Ddp2 carries a 501-bp imperfect inverted repeat, and part of the ori overlaps with one of these repeats. The ori sequence contains two direct repeats of 49 bp comprising two 10-bp "TGTCATGACA" palindromes separated by a poly(T.A) sequence. Deletion of either 49-bp repeat abolished extrachromosomal replication.

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