Cytogenotoxicity in uroepithelial cells of women exposed to mercury in a mining area

Abstract

Objective: To investigate biomarkers of cytogenotoxicity in women exposed to mercury in a mining area. Mercury has been associated with cytogenotoxicity in human and animal models but has not been considered carcinogenic in humans, even though genotoxic effects following exposure to inorganic mercury compounds have been observed.

Methods: A cross-sectional study and micronucleus assay in uroepithelial cells were performed in 104 women (12-84 years of age). First urine void samples were taken to determine creatinine-adjusted mercury levels in urine (HgUCr), to isolate cells and to quantify cytogenetic damage.

Results: The geometric average level for HgUCr was 4.9 microg/g (range, 0.4-85). In the generalised linear model, after controlling for other co-variables, we observed that for each 10 microg/g increase in HgUCr, the OR of developing a genotoxic effect was 2.37 (95% CI 1.79 to 2.84), while for cytotoxic damage in uroepithelial cells the OR was 1.34 (95% CI 1.10 to 1.79). These results suggest a possible association between cytogenotoxicity and HgUCr.

Conclusion: Living in a mining area with exposure to inorganic mercury and having higher mercury levels in urine increased the risk of developing uroepithelial cytogenotoxicity.