Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010:2010:620827.
doi: 10.1155/2010/620827. Epub 2010 Jul 27.

Oral microencapsulated live Saccharomyces cerevisiae cells for use in renal failure uremia: preparation and in vivo analysis

Razek Coussa  1 , Christopher Martoni, Jasmine Bhathena, Aleksandra Malgorzata Urbanska, Satya Prakash
Affiliations

Oral microencapsulated live Saccharomyces cerevisiae cells for use in renal failure uremia: preparation and in vivo analysis

Razek Coussa et al. J Biomed Biotechnol. 2010.

Abstract

Orally administrable alginate-poly-L-lysine-alginate (APA) microcapsules containing live yeast cells was investigated for use in renal failure. At all times, yeast cells remain inside the microcapsules, which are then excreted in the stool. During their gastrointestinal passage, small molecules, like urea, diffuse into the yeast microcapsules where they are hydrolyzed. Orally administrating these microcapsules to uremic rats was found to decrease urea concentrations from 7.29 +/- 0.89 mmol/L to 6.12 +/- 0.90 mmol/L over a treatment period of eight weeks. After stopping the treatment, the urea concentrations increased back to uremic levels of 7.64 +/- 0.77 mmol/L. The analysis of creatinine concentrations averaged 39.19 +/- 4.33 micromol/L, 50.83 +/- 5.55 micromol/L, and 50.28 +/- 7.10 micromol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. While creatinine concentrations for both uremic-control and uremic-treatment groups did not differ among each other (P > .05), they were, however, significantly higher than those of the normal control group (P < .05). Uric acid concentrations averaged 80.08 +/- 26.49 micromol/L, 99.92 +/- 26.55 micromol/L, and 86.49 +/- 28.42 micromol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. There were no significant differences in both calcium and phosphate concentrations among all three groups (P > .05). The microbial populations of five tested types of bacteria were not substantially altered by the presence of the yeast APA encapsulated yeast (P > .05).

PubMed Disclaimer

Figures

Figure 1
Figure 1
Rats' body weight before, during and after oral administration of APA microencapsulated yeast cells.
Figure 2
Figure 2
Plasma urea concentration before, during and after oral administration of APA microencapsulated yeast cells.
Figure 3
Figure 3
Plasma creatinine concentration before, during and after oral administration of APA microencapsulated yeast cells.
Figure 4
Figure 4
Plasma uric acid concentration before, during and after oral administration of APA microencapsulated yeast cells.
Figure 5
Figure 5
Plasma calcium concentration before, during and after oral administration of APA microencapsulated yeast cells.
Figure 6
Figure 6
Plasma phosphate concentration before, during and after oral administration of APA microencapsulated yeast cells.
See this image and copyright information in PMC

References

    1. Brady HR, Clarkson MR, Lieberthal W. Acute renal failure. In: Brenner BM, editor. Brenner & Rector’s the Kidney. Philadelphia, Pa, USA: W. B. Saunders; 2004. pp. 1215–1292.
    1. Coresh J, Astor BC, Greene T, Eknoyan G, Levey AS. Prevalence of chronic kidney disease and decreased kidney function in the adult US population: Third National Health and Nutrition Examination Survey. American Journal of Kidney Diseases. 2003;41(1):1–12. - PubMed
    1. Thadhani R, Pascual M, Bonventre JV. Acute renal failure. The New England Journal of Medicine. 1996;334(22):1448–1460. - PubMed
    1. Lieberthal W, Nigam SK. Acute renal failure. II. Experimental models of acute renal failure: imperfect but indispensable. American Journal of Physiology. 2000;278(1):F1–F12. - PubMed
    1. Nigam SK, Lieberthal W. Acute renal failure. III. The role of growth factors in the process of renal regeneration and repair. American Journal of Physiology. 2000;279(1):F3–F11. - PubMed

Publication types

MeSH terms

LinkOut - more resources